Uptake and metabolism of plasma-derived erucic acid by rat brain

Uptake and metabolism of plasma-derived erucic acid by rat brain.

We examined the ability of erucic acid (22:1n-9) to cross the blood-brain barrier (BBB) by infusing [14-14C]22:1n-9 (170 microCi/kg, iv and icv) into awake, male rats. [1-14C]arachidonic acid (20:4n-6) [intravenous (i.v.)] was the positive control. After i.v. infusion, 0.011% of the plasma [14-14C]22:1n-9 was extracted by the brain, compared with 0.055% of the plasma [1-14C]20:4n-6. The [14-14C...

The absence of ABCD2 sensitizes mice to disruptions in lipid metabolism by dietary erucic acid.

ABCD2 (D2) is a peroxisomal transporter that is highly abundant in adipose tissue and promotes the oxidation of long-chain MUFA. Erucic acid (EA, 22:1ω9) reduces very long chain saturated fatty acids in patients with X-linked adrenoleukodystrophy but promotes dyslipidemia and dilated cardiomyopathy in rats. To determine the role of D2 in the metabolism of EA, we challenged wild-type and D2 defi...

Uptake and Metabolism of Plasma Glutamine by the Small Intestine*

An isolated, vascularly perfused preparation of rat intestine extracted large amounts of glutamine (75 pmoles per hour), but no other amino acid, from a recirculated blood perfusate. With L-[UJ%]glutamine, the carbon was partly incorporated into tissue acid-insoluble material (14%) and the rest reappeared with little delay in intestinal venous blood in COZ (57%), citrulline (6%), proline (5%), ...

Uptake and metabolism of sulphated steroids by the blood–brain barrier in the adult male rat

Little is known about the origin of the neuroactive steroids dehydroepiandrosterone sulphate (DHEAS) and pregnenolone sulphate (PregS) in the brain or of their subsequent metabolism. Using rat brain perfusion in situ, we have found 3 H-PregS to enter more rapidly than 3 H-DHEAS and both to undergo extensive (> 50%) desulphation within 0.5 min of uptake. Enzyme activity for the steroid sulphatas...

Uptake and metabolism of triglycerides by the rat liver.